M7 Medium

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

M7 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract solution.............................................................200.0mL
Dialyzed fetal bovine serum..................................................100.0mL
L -Methionine solution..............................................................30.0mL
Buffer solution.........................................................................20.0mL
Glucose solution ......................................................................20.0mL
Yeast Extract Solution:
Composition per liter:
Yeast extract................................................................................25.0g
Preparation of Yeast Extract Solution:  Add yeast extract to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
L -Methionine Solution:
Composition per liter:
L -Methionine.................................................................................1.5g
Preparation of  L -Methionine Solution:  Add  L -methionine to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Glucose Solution:
Composition per liter:
Glucose .....................................................................................270.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C.
Buffer Solution:
Composition per liter:
Na 2 HPO 4 .................................................................................... 25.0g
KH 2 PO 4 .......................................................................................18.1g
Preparation of Buffer Solution:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Dialyzed Fetal Bovine Serum:
Composition per 100.0mL:
Fetal bovine serum, heat inactivated......................................100.0mL
Preparation of Dialyzed Fetal Bovine Serum: Dialyze the heat-
inactivated serum at 0°–4°C against 10 volumes of water. Clean the di-
alysis tubing before use by boiling in a solution of 0.37g/L of EDTA
and rinsing with water. Change the water four times at 8–16 hr inter-
vals. Centrifuge the dialyzed serum for 30 min at 35,000 x g and filter
sterilize.
Preparation of Medium: Aseptically combine the sterile solutions.
Mix thoroughly. Bring volume to 1.0L with sterile distilled/deionized
water.
Use: For the cultivation of Naegleria fowleri, Naegleria gruberi, and
Nuclearia species.