Acidianus infernus Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidianus infernus Medium
Composition per liter:
(NH 4 ) 2 SO 4 ....................................................................................1.3g
Sulfur flowers...............................................................................1.0g
KH 2 PO 4 .......................................................................................0.28g
MgSO 4 ·7H 2 O..............................................................................0.25g
CaCl 2 ·2H 2 O ................................................................................0.07g
FeCl 3 ·6H 2 O.................................................................................0.02g
Na 2 B 4 O 7 ·10H 2 O.........................................................................4.5mg
MnCl 2 ·4H 2 O ..............................................................................1.8mg
Resazurin...................................................................................1.0mg
ZnSO 4 ·7H 2 O............................................................................0.22mg
CuCl 2 ·2H 2 O.............................................................................0.05mg
Na 2 MoO 4 ·2H 2 O.......................................................................0.03mg
VOSO 4 ·2H 2 O...........................................................................0.03mg
CoSO 4 ......................................................................................0.01mg
Yeast extract solution...............................................................10.0mL
pH 2.5 ± 0.2 at 25°C
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................0.5g
Preparation of Yeast Extract Solution : Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except sulfur flowers
and yeast extract solution, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Al-
low to cool under 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi
pressure–121°C. Sulfur flowers are sterilized separately by steaming
for 3 hr on 3 consecutive days. Aseptically and anaerobically combine
the basal solution, sterile sulfur flowers, and sterile yeast extract solu-
tion. Adjust pH to 2.5 with 6N H 2 SO 4 . Pressurize the culture bottles to
100kPa with 80% N 2 + 20% CO 2 .
Use: For the anaerobic cultivation and maintenance of Acidianus
infernus, Acidianus brierleyi, and Desulfurolobus ambivalens.