Luria HiVeg Agar with Glucose

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Luria HiVeg Agar with Glucose

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Plant hydrolysate ........................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Glucose solution......................................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without glucose solution, is available as a pre-
mixed powder from HiMedia.
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Bring pH to 7.0. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically add 20.0mL of
sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation of Escherichia coli.