Luria Bertani HiVeg Agar, Miller

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Luria Bertani HiVeg Agar, Miller

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Plant hydrolysate.........................................................................10.0g
NaCl............................................................................................10.0g
Yeast extract..................................................................................5.0g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of bacteria for genetic and
molecular studies. For the cultivation and maintenance of recombinant
strains of Escherichia coli for genetic studies.