Luminous Medium

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Luminous Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................30.0g
Agar............................................................................................20.0g
NH 4 Cl ...........................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................3.9g
KH 2 PO 4 .........................................................................................2.1g
CaCO 3 ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
KCl..............................................................................................0.75g
Tris buffer (1M solution, pH 7.5).............................................50.0mL
Glycerol .....................................................................................3.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Alteromonas hanedai,
Photobacterium species, Shewanella hanedai, and Vibrio species.