LTH Medium for Thiothrix with Casitone

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

LTH Medium for Thiothrix with Casitone

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

HEPES (N-[2-hydroxyethyl]piperazine-N′-2-
ethanesulfonic acid) buffer...............................................2.38g
Casitone ........................................................................................1.0g
Sodium lactate...............................................................................1.0g
Na 2 S 2 O 3 ·5H 2 O..............................................................................0.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
K 2 HPO 4 .......................................................................................0.11g
MgSO 4 ·7H 2 O................................................................................0.1g
CaCl 2 ·2H 2 O.................................................................................0.05g
KH 2 PO 4 ....................................................................................85.0mg
EDTA.........................................................................................3.0mg
FeCl 3 ·6H 2 O................................................................................2.0mg
Wolfe’s vitamin solution..........................................................10.0mL
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid .................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Wolfe’s Vitamin Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except Wolfe’s vitamin
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Adjust pH to 7.3 with NaOH. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 10.0mL of sterile Wolfe’s vita-
min solution. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the cultivation of unidentified bacterium ATCC 49750.