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培养基配方
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LST-MUG Broth
[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]
LST-MUG Broth
Lactose..........................................................................................5.0g
K 2 HPO 4 ......................................................................................2.75g
KH 2 PO 4 ......................................................................................2.75g
NaCl..............................................................................................5.0g
L -Tryptophan ................................................................................1.0g
Sodium lauryl sulfate....................................................................0.1g
4-Methylumbelliferyl-β- D -glucuronide........................................0.1g
pH 6.8 ± 0.2 at 37°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube in 10.0mL volumes. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use : For the detection of E. coli by the fluorogenic method. The pres-
ence of E. coli results in fluorescence in the UV. A positive indole test
provides confirmation. β-D-glucoronidase, which is produced by E.
coli, cleaves 4-methylumbelliferyl-β-D-glucuronide to 4-methylum-
belliferone and glucuronide. The fluorogen 4-methylumbelliferone can
be detected under a long wavelength UV lamp.
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Tryptose......................................................................................20.0gLactose..........................................................................................5.0g
K 2 HPO 4 ......................................................................................2.75g
KH 2 PO 4 ......................................................................................2.75g
NaCl..............................................................................................5.0g
L -Tryptophan ................................................................................1.0g
Sodium lauryl sulfate....................................................................0.1g
4-Methylumbelliferyl-β- D -glucuronide........................................0.1g
pH 6.8 ± 0.2 at 37°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube in 10.0mL volumes. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use : For the detection of E. coli by the fluorogenic method. The pres-
ence of E. coli results in fluorescence in the UV. A positive indole test
provides confirmation. β-D-glucoronidase, which is produced by E.
coli, cleaves 4-methylumbelliferyl-β-D-glucuronide to 4-methylum-
belliferone and glucuronide. The fluorogen 4-methylumbelliferone can
be detected under a long wavelength UV lamp.