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LS Differential HiVeg Medium Base with Skim Milk and Triphenyltetrazolium Chloride
[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]
LS Differential HiVeg Medium Base
with Skim Milk and Triphenyltetrazolium Chloride
Agar ............................................................................................15.0g
Plant hydrolysate.........................................................................10.0g
Plant extract ..................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
L -Cysteine·HCl..............................................................................0.3g
Skim milk solution.................................................................100.0mL
Triphenyltetrazolium chloride solution....................................10.0mL
pH 6.1 ± 0.2 at 25°C
Source: This medium, without skim milk and triphenyltetrazolium
chloride solution, is available as a premixed powder from HiMedia.
Skim Milk Solution:
Composition per 100.0mL:
Skim milk, antibiotic free ...........................................................10.0g
Preparation of Skim Milk Solution: Add skim milk to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 5 min at 15 psi pressure–121°C. Cool to 50°C.
Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium chloride.............................................0.2g
Preparation of Triphenyltetrazolium Chloride Solution:
Add triphenyltetrazolium chloride to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Warm to
50°C.
Preparation of Medium: Add components, except skim milk solu-
tion and triphenyltetrazolium chloride solution, to distilled/deionized
water and bring volume to 890.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile skim milk solution and sterile triphe-
nyltetrazolium chloride solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the differentiation and enumeration of lactobacilli and strepto-
cocci in yogurt. Lactobacillus species appear as irregular, red colonies
surrounded by a white, opaque zone. Streptococcus species appear as
round, red colonies surrounded by a clear zone.
with Skim Milk and Triphenyltetrazolium Chloride
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Glucose .......................................................................................20.0gAgar ............................................................................................15.0g
Plant hydrolysate.........................................................................10.0g
Plant extract ..................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
L -Cysteine·HCl..............................................................................0.3g
Skim milk solution.................................................................100.0mL
Triphenyltetrazolium chloride solution....................................10.0mL
pH 6.1 ± 0.2 at 25°C
Source: This medium, without skim milk and triphenyltetrazolium
chloride solution, is available as a premixed powder from HiMedia.
Skim Milk Solution:
Composition per 100.0mL:
Skim milk, antibiotic free ...........................................................10.0g
Preparation of Skim Milk Solution: Add skim milk to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 5 min at 15 psi pressure–121°C. Cool to 50°C.
Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium chloride.............................................0.2g
Preparation of Triphenyltetrazolium Chloride Solution:
Add triphenyltetrazolium chloride to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Warm to
50°C.
Preparation of Medium: Add components, except skim milk solu-
tion and triphenyltetrazolium chloride solution, to distilled/deionized
water and bring volume to 890.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile skim milk solution and sterile triphe-
nyltetrazolium chloride solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the differentiation and enumeration of lactobacilli and strepto-
cocci in yogurt. Lactobacillus species appear as irregular, red colonies
surrounded by a white, opaque zone. Streptococcus species appear as
round, red colonies surrounded by a clear zone.
