LPBM Acido-Thermophile Medium

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

LPBM Acido-Thermophile Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Cellulose.......................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
Cellobiose.....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Na 2 HPO 4 ·7H 2 O.............................................................................0.1g
CaCl 2 ·2H 2 O ................................................................................0.02g
pH 5.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except cellulose and
cellobiose, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Adjust pH to 5.2 with H 3 PO 4 . Add cellulose and cellobio-
se. Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Acidothermus cellulolyt-
icus.