Low Iron YC Broth

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Low Iron YC Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1033.0mL:

Solution 1......................................................................................1.0L
Solution 4.................................................................................30.0mL
Solution 2...................................................................................2.0mL
Solution 3...................................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Solution 1:
Composition per liter:
Yeast extract................................................................................20.0g
Casamino acids...........................................................................10.0g
KH 2 PO 4 .........................................................................................5.0g
CaCl 2 ·2H 2 O...................................................................................1.0g
Tryptophan..................................................................................0.05g
Preparation of Solution 1:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Gen-
tly heat and bring to boiling. Filter through #40 ashless filter paper.
Solution 2:
Composition per 100.0mL:
MgSO 4 ·7H 2 O..............................................................................22.5g
CuSO 4 ·5H 2 O.................................................................................0.5g
ZnSO 4 ·5H 2 O.................................................................................0.2g
β-Alanine..................................................................................0.115g
Nicotinic acid............................................................................0.115g
MnCl 2 ·4H 2 O..............................................................................0.075g
Pimelic acid................................................................................7.5mg
HCl, concentrated ......................................................................3.0mL
Preparation of Solution 2:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Solution 3:
Composition per 100.0mL:
L- Cystine .....................................................................................20.0g
HCl, concentrated ....................................................................20.0mL
Preparation of Solution 3:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Solution 4:
Composition per 100.0mL:
Maltose .......................................................................................50.0g
CaCl 2 ·2H 2 O ..................................................................................0.5g
Preparation of Solution 4:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 10
min at 11 psi pressure–116°C. Cool to 25°C.
Preparation of Medium:  To 1.0L of solution 1, add 2.0mL of so-
lution 2 and 1.0mL of solution 3. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 30.0mL
of sterile solution 4. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of Corynebacterium diph-
theriae.