Loeffler Slant, Modified

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Loeffler Slant, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .........................................................................................1.0g
Peptone..........................................................................................0.5g
Beef serum.............................................................................300.0mL
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add peptone and glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Add beef
serum. Mix thoroughly. Adjust pH to 7.6. Distribute into screw-capped
tubes in 3.0mL volumes. Slant tubes in the autoclave. Autoclave for 30
min at 0 psi pressure–100°C.
Use: For the cultivation of Corynebacterium diphtheriae. For demon-
stration of pigment production and proteolysis by Corynebacterium
diphtheriae. For the cultivation and maintenance of Moraxella lacu-
nata.