Loefer's Medium

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Loefer's Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone.........................................................................15.0g
Casitone ........................................................................................5.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................2.0g
Na 2 HPO 4 .......................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Yeast extract..................................................................................0.5g
MgCl 2 ............................................................................................0.3g
Asolectin solution....................................................................10.0mL
Asolectin Solution:
Asolectin.......................................................................................1.0g
Ethanol...................................................................................100.0mL
Preparation of Asolectin Solution: Add 1.0g of asolectin to eth-
anol and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Distribute into screw-
capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Tetrahymena patula.