Liver Infusion Sake Medium

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Liver Infusion Sake Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Liver, fresh................................................................................400.0g
Agar............................................................................................15.0g
Sake........................................................................................400.0mL
Preparation of Medium: Finely mince liver and add to 600.0mL of
distilled/deionized water. Gently heat and bring to boiling. Continue
boiling for 5–10 min. Filter through Whatman #2 filter paper. To fil-
trate, add agar and sake. Bring volume to 1.0L with distilled/deionized
water. Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 25 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus fructiv-
orans and Lactobacillus homohiochi.