Littman Oxgall HiVeg Agar Base with Streptomycin

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Littman Oxgall HiVeg Agar Base with Streptomycin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Plant peptone...............................................................................20.0g
Glucose .......................................................................................10.0g
Synthetic detergent No. II.............................................................5.0g
Crystal Violet..............................................................................0.01g
Streptomycin solution..............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium, without streptomycin, is available as a pre-
mixed powder from HiMedia.
Streptomycin Solution:
Composition per 10.0mL:
Streptomycin...............................................................................0.03g
Preparation of Streptomycin Solution: Add streptomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except streptomycin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile strepto-
mycin solution. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes. Allow tubes to cool in a slanted position.
Use: For the selective isolation and cultivation of fungi, especially
dermatophytes.