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Listeria Enrichment Broth II, USDA FSIS (Listeria Primary Selective Enrichment Broth, UVM II) (University of Vermont II Listeria Primary Selective Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Enrichment Broth II,
USDA FSIS
(Listeria Primary Selective
Enrichment Broth, UVM II)
(University of Vermont II Listeria
Primary Selective Enrichment Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
Na 2 HPO 4 .....................................................................................12.0g
Beef extract...................................................................................5.0g
Protease peptone...........................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .......................................................................................1.35g
Esculin ..........................................................................................1.0g
Nalidixic acid solution...............................................................1.0mL
Acriflavine solution ...................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid................................................................................0.2g
NaOH (0.1M solution).............................................................10.0mL
Preparation of Nalidixic Acid Solution: Add nalidixic acid to
10.0mL of NaOH solution. Mix thoroughly. Filter sterilize.
Acriflavine Solution:
Composition per 10.0mL:
Acriflavine..................................................................................0.25g
Preparation of Acriflavine Solution: Add acriflavine to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize. Use freshly prepared solution.
Preparation of Medium: Add components, except nalidixic acid
solution and acriflavine solution, to distilled/deionized water and bring
volume to 998.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 1.0mL of sterile nalidixic acid solution. Mix thorough-
ly. Store at 4°C. Immediately prior to use, aseptically add 1.0mL of
sterile acriflavine solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the selective isolation, cultivation, and enrichment of Listeria
monocytogenes from food, milk, and dairy products.

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Listeria Enrichment Broth II, USDA FSIS (Listeria Primary Selective Enrichment Broth, UVM II) (University of Vermont II Listeria Primary Selective Enrichment Broth