Lipovitellin Salt Mannitol Agar

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Lipovitellin Salt Mannitol Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................75.0g
Egg yolk......................................................................................20.0g
Agar............................................................................................15.0g
D- Mannitol ..................................................................................10.0g
Polypeptone™ ............................................................................10.0g
Beef extract...................................................................................1.0g
Phenol Red................................................................................0.025g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the detection of Staphylococcus aureus in swimming pool
water based on lipovitellin-lipase activity and mannitol fermentation.
Staphylococcus aureus and other bacteria with lipovitellin-lipase activity
attack the egg yolk and appear as colonies surrounded by an opaque
zone. Bacteria that ferment mannitol appear as colonies surrounded by a
yellow zone.