LHET2 Medium

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

LHET2 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL
Solution B..............................................................................500.0mL
pH 2.5–3.0 at 25°C
Solution A:
Composition per 500.0mL:
(NH 4 ) 2 SO 4 .....................................................................................2.0g
K 2 HPO 4 .......................................................................................0.51g
MgSO 4 ·7H 2 O................................................................................0.5g
KCl................................................................................................0.1g
Pancreatic digest of casein..........................................................0.06g
NaCl............................................................................................0.02g
Papaic digest of soybean meal....................................................0.01g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Adjust pH to 2.5–3.0 with 1N H 2 SO 4 . Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution B:
Composition per 500.0mL:
Agar ............................................................................................12.0g
Glucose .........................................................................................1.0g
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Preparation of Medium: Aseptically combine sterile solution A
and sterile solution B. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use:  For the cultivation and maintenance of Acidiphilium cryptum.