Leucothrix Medium (ATCC Medium 430)

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Leucothrix Medium
(ATCC Medium 430)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................11.7g
MgCl 2 ·6H 2 O................................................................................5.35g
Na 2 SO 4 ..........................................................................................2.0g
CaCl 2 ·2H 2 O.................................................................................0.75g
Pancreatic digest of casein............................................................0.5g
Yeast extract..................................................................................0.5g
Tris(hydroxymethyl)aminomethane buffer...................................0.5g
KCl..............................................................................................0.35g
Na 2 HPO 4 .....................................................................................0.05g
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Leucothrix mucor.