Leucothrix Medium

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Leucothrix Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
Synthetic seawater...............................................................1000.0mL
pH 7.8 ± 0.2 at 25°C
Synthetic Seawater:
Composition per liter:
NaCl............................................................................................24.0g
MgCl 2 ·6H 2 O ............................................................................... 11.0g
Na 2 SO 4 ..........................................................................................4.0g
CaCl 2 ·6H 2 O ..................................................................................2.0g
KCl................................................................................................0.7g
KBr ...............................................................................................0.1g
SrCl 2 ·6H 2 O .................................................................................0.04g
H 3 BO 3 .........................................................................................0.03g
NaSiO 3 ·9H 2 O............................................................................ 5.0mg
NaF ............................................................................................3.0mg
NH 4 NO 3 .....................................................................................2.0mg
FePO 4 ·4H 2 O.............................................................................. 1.0mg
Preparation of Synthetic Seawater:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add 10.0g of pancreatic digest of casein to
1.0L of synthetic seawater. Mix thoroughly. Adjust pH to 7.8. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Leucothrix species.