Leuconostoc oenos Medium

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Leuconostoc oenos Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................10.0g
Peptone........................................................................................10.0g
Yeast extract..................................................................................5.0g
MnSO 4 ·4H 2 O................................................................................0.1g
Tomato juice...........................................................................250.0mL
L -Cysteine·HCl solution...........................................................10.0mL
pH 4.8 ± 0.2 at 25°C
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl..............................................................................0.5g
Preparation of  L -Cysteine·HCl Solution: Add  L -cysteine·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except  L -cysteine·HCl
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C. Aseptically add sterile  L -
cysteine·HCl solution. Mix thoroughly. Aseptically distribute into ster-
ile tubes or flasks.
Use: For the cultivation of Leuconostoc oenos.