Leptothrix 2X PYG Medium

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptothrix 2X PYG Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

HEPES (N-2-Hhydroxyethyl piperazine-N′-
2-ethanesulfonic acid) buffer............................................3.57g
MgSO 4 ·7H 2 O................................................................................0.6g
Glucose.........................................................................................0.5g
Peptone .........................................................................................0.5g
Yeast extract..................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.07g
MnSO 4 ·H 2 O..............................................................................0.017g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation and maintenance of Leptothrix discophora.