Leishmania Medium

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leishmania Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Sodium citrate...............................................................................1.2g
NaCl..............................................................................................1.0g
Rabbit blood solution...............................................................10.0mL
Rabbit Blood Solution:
Composition per 10.0mL:
Rabbit blood, defibrinated.........................................................5.0mL
Preparation of Rabbit Blood Solution: Add 5.0mL of whole
rabbit blood to 5.0mL of sterile distilled/deionized water. Freeze and
thaw twice to lyse blood cells.
Preparation of Medium: Add components, except rabbit blood so-
lution, to distilled/deionized water and bring volume to 90.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical-
ly add 10.0mL of sterile rabbit blood solution. Mix thoroughly. Asep-
tically distribute into sterile, screw-capped tubes or flasks.
Use: For the cultivation of Leishmania donovani, Leishmania hertigi,
and Leishmania tropica.