Legionella Agar Base (Legionella Medium) (BCYEα Agar, Modified)

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Legionella Agar Base
(Legionella Medium)
(BCYEα Agar, Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................17.0g
Yeast extract................................................................................10.0g
ACES buffer (N-2-acetamido-
2-aminoethane sulfonic acid) .................................................6.0g
Charcoal, activated........................................................................1.5g
KOH..............................................................................................1.5g
α-Ketoglutarate.............................................................................1.0g
Legionella agar enrichment .....................................................10.0mL
pH 6.85–7.0 at 25°C
Source:  This medium is available as a prepared medium from BD Di-
agnostic Systems.
Legionella Agar Enrichment:
Composition per 10.0mL:
L- Cysteine·HCl·H 2 O......................................................................0.4g
Fe 4 (P 2 O 7 ) 3 ...................................................................................0.25g
Preparation of Legionella Agar Enrichment: Add components
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except Legionella agar
enrichment, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50° C. Add 10.0mL of sterile Legionella agar
enrichment. Adjust pH to 6.9 at 50°C by adding 4.0–4.5mL of 1.0N
KOH. This is a critical step. Mix thoroughly. Pour into sterile Petri dishes
in 20.0mL volumes. Swirl medium while pouring to keep charcoal in
suspension.
Use: For the preparation of Legionella agars. For the isolation and cul-
tivation of Legionella species from clinical and nonclinical materials.