LBE Medium

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

LBE Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Glucose solution ......................................................................10.0mL
50X medium E...........................................................................4.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
D- Glucose....................................................................................20.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
50X Medium E:
Composition per liter:
K 2 HPO 4 , anhydrous..................................................................500.0g
Na(NH 4 )HPO 4 ·4H 2 O ................................................................175.0g
Citric acid·H 2 O..........................................................................100.0g
MgSO 4 ·7H 2 O..............................................................................10.0g
Preparation of 50X Medium E: Add components to 670.0mL of
distilled/deionized water in the following order: MgSO 4 ·7H 2 O, citric
acid·H 2 O, K 2 HPO 4 , and Na(NH 4 )HPO 4 ·4H 2 O. Mix thoroughly. Bring
volume to 1.0L with distilled/deionized water.
Preparation of Medium: Add components—except glucose solu-
tion and 50X medium E—to distilled/deionized water and bring vol-
ume to 986.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile
glucose solution and 4.0mL of sterile 50X medium E. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Escherichia coli.