Lange Medium

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lange Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Maltose .........................................................................................5.0g
Ca(NO 3 ) 2 .......................................................................................0.5g
MgSO 4 ..........................................................................................0.5g
K 2 HPO 4 .......................................................................................0.25g
Peptone .........................................................................................0.1g
Horse dung extract.................................................................100.0mL
Horse Dung Extract:
Composition per liter:
Horse dung, fresh......................................................................100.0g
Preparation of Horse Dung Extract: Add fresh horse dung to
distilled/deionized water and bring volume to 1.0L. Autoclave for 50
min at 15 psi pressure–121°C. Filter through Whatman #1 filter paper.
Reserve filtrate.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Coprinus xanthothrix.