Lactose Sulfite Broth Base

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lactose Sulfite Broth Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Lactose........................................................................................10.0g
Casein enzymic hydrolysate.........................................................5.0g
Yeast extract..................................................................................2.5g
NaCl..............................................................................................2.5g
L -Cysteine·HCl·H 2 O.....................................................................0.3g
Sodium metabisulfite solution.................................................50.0mL
Ferric ammonium citrate..........................................................50.0mL
pH 7.1 ± 0.2 at 25°C

Source: This medium is available from HiMedia.

Selective Sodium Metabisulfite Solution:
Composition per 100.0mL:
Sodium metabisulfite....................................................................1.2g
Preparation of Sodium Metabisulfite Solution:  Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Selective Ferric Ammonium Citrate Solution:
Composition per 100.0mL:
Ferric ammonium citrate...............................................................1.0g
Preparation of Ferric Ammonium Citrate Solution:  Add
components to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sodium meta-
bisulfite and ferric ammonium citrate solutions, to distilled/deionized
water and bring volume to 999.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add sodium
metabisulfite and ferric ammonium citrate solutions. Mix thoroughly.
Pour into Petri dishes or aseptically distribute into sterile tubes.
Use: For the detection and enumeration of Clostridium perfringens in
pharmaceutical products.