Lactose Gelatin Medium

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lactose Gelatin Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin.......................................................................................120.0g
Tryptose ......................................................................................15.0g
Lactose........................................................................................10.0g
Yeast extract................................................................................10.0g
Phenol Red (0.5% solution).....................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add tryptose, yeast extract, and lactose
to distilled/deionized water and bring volume to 400.0mL. Mix thor-
oughly. Add gelatin to distilled/deionized water and bring volume to
590.0mL. Gently heat gelatin solution while stirring and bring to 50°–
60°C. Add Phenol Red. Mix the two solutions together. Distribute into
tubes in 10.0mL volumes. Autoclave for 10 min at 15 psi pressure–
121°C. If medium is not used in 8 hr, deoxygenate by heating to 50°–
70°C for 2–3 hr prior to inoculation.
Use: For the cultivation of Clostridium perfringens.