Lactose Blue Agar (B.T.B. Lactose Agar, Modified)

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lactose Blue Agar
(B.T.B. Lactose Agar, Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Lactose .......................................................................................15.5g
Agar ............................................................................................13.0g
NaCl..............................................................................................5.0g
Peptic digest of animal tissue....................................................... 3.5g
Casein enzymic hydrolysate ........................................................3.5g
Bromthymol Blue ......................................................................0.04g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes.
Use: For the differentiation of lactose-fermenting and non-fermenting
bacteria belonging to Enterobacteriaceae.