Lactic Agar for Yogurt Bacteria, Modified

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactic Agar for Yogurt Bacteria, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Elliker agar....................................................................................1.0L
Milk solution............................................................................70.0mL
pH 6.8 ± 0.1 at 25°C
Elliker Agar:
Composition per liter:
Pancreatic digest of casein..........................................................20.0g
Agar ............................................................................................15.0g
Yeast extract................................................................................10.0g
Gelatin...........................................................................................4.0g
Glucose .........................................................................................3.0g
Ascorbic acid ................................................................................2.5g
Lactose..........................................................................................2.5g
NaCl..............................................................................................2.5g
Sodium acetate..............................................................................2.5g
Sucrose..........................................................................................2.5g
Preparation of Elliker Agar: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Milk Solution:
Composition per 100.0mL:
Nonfat dry milk solids ................................................................11.0g
Preparation of Milk Solution:  Add nonfat dry milk solids to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 12 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium:  Add 70.0mL of sterile milk solution to
1.0L of cooled, sterile Elliker agar. Mix thoroughly. Pour into sterile
Petri dishes. Allow plates to dry at 28°–30°C for 18–24 hr.
Use: For the cultivation of acidogenic microorganisms, especially
Lactobacillus species and lactic streptococci, from foods.