Lachica’s Medium Base

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lachica’s Medium Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from..........................................................500.0g
Agar............................................................................................15.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Amylose Azure.............................................................................3.0g
Selective supplement solution .................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Ampicillin................................................................................10.0mg
Preparation of Selective Supplement Solution:  Add ampicillin
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the isolation and cultivation of Aeromonas hydrophila from
foods stored under different temperature conditions.