L Medium with Methanol

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

L Medium with Methanol

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
NaNO 3 ..........................................................................................2.0g
Na 2 HPO 4 .....................................................................................0.21g
MgSO 4 ·7H 2 O................................................................................0.2g
NaH 2 PO 4 .....................................................................................0.09g
KCl..............................................................................................0.04g
CaCl 2 .........................................................................................0.015g
FeSO 4 ·7H 2 O...............................................................................1.0mg
Methanol..................................................................................20.0mL
Salts solution..............................................................................1.0mL
Salts Solution:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O..............................................................................7.0mg
H 3 BO 3 ........................................................................................1.0mg
MnSO 4 ·5H 2 O.............................................................................1.0mg
MoO 3 ..........................................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................0.5mg
Preparation of Salts Solution: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 980.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Filter sterilize methanol. Asepti-
cally add 20.0mL of sterile methanol to cooled, sterile basal medium.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation and maintenance of Methylobacillus glyco-
genes.