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L Medium with DAP and THY (L Medium with Diaminopimelic Acid and Thymidine)
[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]
L Medium with DAP and THY
(L Medium with Diaminopimelic Acid and Thymidine)
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Glucose solution ......................................................................10.0mL
Diaminopimelic acid solution..................................................10.0mL
Thymidine solution..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................1.0g
Preparation of Glucose Solution: Add D- glucose to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Diaminopimelic Acid Solution:
Composition per 10.0mL:
DL- Diaminopimelic acid................................................................0.1g
Preparation of Diaminopimelic Acid Solution: Add diamin-
opimelic acid to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Thymidine Solution:
Composition per 10.0mL:
Thymidine...................................................................................0.02g
Preparation of Thymidine Solution: Add thymidine to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except glucose solu-
tion, diaminopimelic acid solution, and thymidine solution—to dis-
tilled/deionized water and bring volume to 970.0mL. Mix thoroughly.
Gently heat and bring to boiling. Adjust pH to 7.0. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add ster-
ile glucose solution, diaminopimelic acid solution, and thymidine so-
lution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation and maintenance of Escherichia coli.
(L Medium with Diaminopimelic Acid and Thymidine)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of casein..........................................................10.0gNaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Glucose solution ......................................................................10.0mL
Diaminopimelic acid solution..................................................10.0mL
Thymidine solution..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................1.0g
Preparation of Glucose Solution: Add D- glucose to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Diaminopimelic Acid Solution:
Composition per 10.0mL:
DL- Diaminopimelic acid................................................................0.1g
Preparation of Diaminopimelic Acid Solution: Add diamin-
opimelic acid to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Thymidine Solution:
Composition per 10.0mL:
Thymidine...................................................................................0.02g
Preparation of Thymidine Solution: Add thymidine to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except glucose solu-
tion, diaminopimelic acid solution, and thymidine solution—to dis-
tilled/deionized water and bring volume to 970.0mL. Mix thoroughly.
Gently heat and bring to boiling. Adjust pH to 7.0. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add ster-
ile glucose solution, diaminopimelic acid solution, and thymidine so-
lution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation and maintenance of Escherichia coli.
