L and F Basal Salts, Modified with Heptadecane

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

L and F Basal Salts, Modified with Heptadecane

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NH 4 Cl ...........................................................................................2.0g
Na 2 HPO 4 .....................................................................................0.21g
MgSO 4 ·7H 2 O................................................................................0.2g
NaH 2 PO 4 .....................................................................................0.09g
KCl..............................................................................................0.04g
CaCl 2 .........................................................................................0.015g
FeSO 4 ·7H 2 O...............................................................................1.0mg
ZnSO 4 ·7H 2 O............................................................................0.07mg
H 3 BO 3 ......................................................................................0.01mg
MnSO 4 ·5H 2 O...........................................................................0.01mg
MoO 3 ........................................................................................0.01mg
CuSO 4 ·5H 2 O...............................................................................5.0μg
Heptadecane...............................................................................2.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except heptadecane, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute equally into four 250.0mL
volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
60°C. Filter sterilize heptadecane. To one 250.0mL fraction of basal
salts, aseptically add 0.5mL of sterile heptadecane. Pour mixture into a
sterile blender. Homogenize slowly to mix heptadecane with basal salts
and not to create excess bubbles. Rapidly distribute medium to sterile
screw-capped tubes. Chill tubes quickly in an ice pack or in the refrig-
erator. Allow tubes to solidify in a slanted position.
Use:  For the cultivation and maintenance of Thermoleophilum album
and Thermoleophilum minutum.