L Broth DAP Thymidine

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

L Broth DAP Thymidine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Diaminopimelic acid solution..................................................10.0mL

Thymidine solution..................................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Diaminopimelic Acid Solution:
Composition per 10.0mL:
Diaminopimelic acid.....................................................................0.1g
Preparation of Diaminopimelic Acid Solution: Add diamin-
opimelic acid to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Thymidine Solution:
Composition per 10.0mL:
Thymidine...................................................................................0.01g
Preparation of Thymidine Solution: Add thymidine to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except diaminopimelic
acid solution and thymidine solution, to distilled/deionized water and
bring volume to 980.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave
for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile
diaminopimelic acid solution and 10.0mL of sterile thymidine solu-
tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.