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Kupferberg Trichomonas HiVeg Broth Base with Serum and Selective Supplement (Trichomonas HiVeg Broth Base, Kupferberg)
[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]
Kupferberg Trichomonas HiVeg Broth Base
with Serum and Selective Supplement
(Trichomonas HiVeg Broth Base, Kupferberg)
Agar..............................................................................................1.0g
L -Cysteine·HCl .............................................................................1.5g
Maltose .........................................................................................1.0g
Methylene Blue..........................................................................3.0mg
Bovine serum...........................................................................50.0mL
Selective supplement...............................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Source: This medium, without bovine serum and selective supple-
ment, is available as a premixed powder from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Chloramphenicol...........................................................................1.0g
Preparation of Selective Supplement Solution: Add chloram-
phenicol to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except bovine serum
adn selective supplement, to distilled/deionized water and bring vol-
ume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 50.0mL of bovine serum and 10.0mL selective supple-
ment.
Use: For the cultivation of Trichomonas species from clinical speci-
mens.
with Serum and Selective Supplement
(Trichomonas HiVeg Broth Base, Kupferberg)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Plant hydrolysate ........................................................................20.0gAgar..............................................................................................1.0g
L -Cysteine·HCl .............................................................................1.5g
Maltose .........................................................................................1.0g
Methylene Blue..........................................................................3.0mg
Bovine serum...........................................................................50.0mL
Selective supplement...............................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Source: This medium, without bovine serum and selective supple-
ment, is available as a premixed powder from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Chloramphenicol...........................................................................1.0g
Preparation of Selective Supplement Solution: Add chloram-
phenicol to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except bovine serum
adn selective supplement, to distilled/deionized water and bring vol-
ume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 50.0mL of bovine serum and 10.0mL selective supple-
ment.
Use: For the cultivation of Trichomonas species from clinical speci-
mens.
