KPL Medium

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

KPL Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1141.0mL:

Agar............................................................................................15.0g
Galactose.....................................................................................10.0g
Glucose.......................................................................................10.0g
Lactic acid whey...........................................................................1.0L
White table wine....................................................................140.0mL
Tween™ 80................................................................................1.0mL
pH 5.5 ± 0.2 at 25°C
Lactic Acid Whey:
Composition per liter:
Skim milk (10% solution).............................................................1.0L
Preparation of Lactic Acid Whey: Adjust the pH of the skim
milk to 5.5 with lactic acid. Gently heat and bring to boiling. Continue
boiling for 30 min. Filter through Whatman #1 filter paper.
Preparation of Medium: Combine components, except white table
wine. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to
5.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Filter sterilize white table wine. To cooled, sterile basal medium, asep-
tically add sterile white table wine. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Lactobacillus kefiranofa-
ciens.