Kohn Two Tube Medium No. 1 Base with Urea

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

Kohn Two Tube Medium No. 1 Base with Urea

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................16.0g
Peptic digest of animal tissue......................................................15.0g
Mannitol......................................................................................10.0g
Beef extract...................................................................................2.0g
Yeast extract..................................................................................2.0g
Glucose .........................................................................................1.0g
Phenol Red..................................................................................0.05g
Urea solution............................................................................25.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without urea solution, is available as a pre-
mixed powder from HiMedia.
Urea Solution:
Composition per 100.0mL:
Urea...............................................................................................5.0g
Preparation of Urea Solution: Add urea to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes. Autoclave for 15 min at 10 psi pressure–
115°C. Cool to 60°C. Aseptically add 25mL of sterile urea solution.
Mix thoroughly. Allow to solidify as a slant in tubes with a genrous
butt.
Use: For the identification of Enterobacteriaceae on the basis of glu-
cose and mannitol fermentation and urease production.