KF Streptococcus Agar

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

KF Streptococcus Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Agar ............................................................................................20.0g
Maltose........................................................................................20.0g
Proteose peptone.........................................................................10.0g
Sodium glycerophosphate...........................................................10.0g
Yeast extract................................................................................10.0g
NaCl..............................................................................................5.5g
Lactose..........................................................................................1.0g
NaN 3 .............................................................................................0.4g
Bromcresol Purple ....................................................................0.015g
2,3,5-Triphenyltetrazolium chloride solution..........................10.0mL
pH 7.2 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
2,3,5-Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium chloride.............................................0.1g
Preparation of 2,3,5-Triphenyltetrazolium Chloride Solu-
tion: Add 2,3,5-triphenyltetrazolium chloride to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except 2,3,5-triphe-

nyltetrazolium chloride solution, to distilled/deionized water and bring

volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 2,3,5-triphenyltetrazolium chloride solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and enumeration of enterococci.