Ketolactonate Broth

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

Ketolactonate Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Lactose........................................................................................10.0g
Yeast extract................................................................................10.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For use in the identification of agrobacteria and other bacteria
based upon production of 3-ketogluconate. After incubation, Bene-

dict’s solution is added to the plates. Yellow zones around colonies

indicate positive production of 3-ketogluconate.