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Keister's Modified TYI-S-33 Medium

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]
Keister's Modified TYI-S-33 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................20.0g
Glucose.......................................................................................10.0g
Yeast extract................................................................................10.0g
L -Cysteine·HCl .............................................................................2.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
Bovine bile..................................................................................0.75g
KH 2 PO 4 .........................................................................................0.6g
Ascorbic acid................................................................................0.2g
Ferric ammonium citrate..........................................................22.8mg
Bovine serum, heat inactivated..............................................100.0mL
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
add 1.0L of sterile, heat-inactivated bovine serum. Mix thoroughly.
Aseptically distribute into sterile, screw-capped tubes or flasks.
Use: For the cultivation of Giardia cati, Giardia intestinalis, and Hex-
amita species.
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