KCN Broth

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

KCN Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................5.64g
NaCl..............................................................................................5.0g
Peptone..........................................................................................3.0g
KH 2 PO 4 .....................................................................................0.225g
KCN (0.5% solution)...............................................................15.0mL
pH 7.6 ± 0.2 at 25°C
Caution: Cyanide is toxic.
Preparation of Medium: Add components, except KCN solution,
to distilled/deionized water and bring volume to 985.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add KCN solution. Mix thoroughly. Aseptically distribute
into sterile tubes. Stopper immediately.
Use: For the differentiation of Enterobacteriaceae based upon growth in the
presence of potassium cyanide.