Kanamycin Vancomycin Laked Blood Agar

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

Kanamycin Vancomycin Laked Blood Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................17.5g
Pancreatic digest of casein..........................................................15.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Kanamycin................................................................................0.075g
Sheep blood, laked...................................................................50.0mL
Vancomycin solution ...............................................................10.0mL
Vitamin K 1 solution...................................................................1.0mL
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin...............................................................................7.5mg
Preparation of Vancomycin Solution: Add vancomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: The blood is laked (hemolyzed) by freez-
ing whole blood overnight and then thawing. Add components, except
sheep blood, vancomycin solution, and vitamin K 1 solution, to dis-
tilled/deionized water and bring volume to 939.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add sheep blood, vanco-
mycin solution, and 1.0mL of vitamin K 1 solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For isolation of the Bacteroides melaninogenicus group.