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K101 Flexibacter Medium

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]
K101 Flexibacter Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
Casamino acids.............................................................................1.0g
Glucose.........................................................................................1.0g
Tris(hydroxymethyl)aminomethane buffer...................................1.0g
CaCl 2 .............................................................................................0.1g
KNO 3 ............................................................................................0.1g
MgSO 4 ·7H 2 O................................................................................0.1g
Sodium glycerophosphate.............................................................0.1g
Thiamine·HCl............................................................................1.0mg
Cyanocobalamin.........................................................................1.0μg
Trace elements solution HO-LE................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Trace Elements Solution HO-LE:
Composition per liter:
H 3 BO 3 .........................................................................................2.85g
MnCl 2 ·4H 2 O .................................................................................1.8g
Sodium tartrate............................................................................1.77g
FeSO 4 ·7H 2 O................................................................................1.36g
CoCl 2 ·6H 2 O................................................................................0.04g
CuCl 2 ·2H  2 O.............................................................................0.027g
Na 2 MoO 4 ·2H 2 O........................................................................0.025g
ZnCl 2 ...........................................................................................0.02g
Preparation of Trace Elements Solution HO-LE: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except trace elements
solution HO-LE, to distilled/deionized water and bring volume to
999.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 1.0mL of trace elements solution HO-LE. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Cytophaga species, Flex-
ibacter species, Herpetosiphon geysericola, and Myxococcus fulvus.
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