Jones–Kendrick Pertussis Transport Medium

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

Jones–Kendrick
Pertussis Transport Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion................................................500.0g
Agar ............................................................................................20.0g
Soluble starch..............................................................................10.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Charcoal powder, activated...........................................................4.0g
Yeast extract..................................................................................3.5g
Penicillin solution....................................................................10.0mL

pH 7.4 ± 0.2 at 25°C

Penicillin Solution:

Composition per 10.0mL:
Penicillin..................................................................................... 300U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except penicillin solu-
tion, starch, yeast extract, heart infusion, and agar, to water. Boil to dis-
solve. Add charcoal, mix well, and autoclave. Cool to 50°C, add
penicillin, and dispense into small bottles as slants. Cool and seal tight-
ly. Store at 5°C. Stable for 2 to 3 months.

Use: For the cultivation and transport of Bordetella pertussis between

clinical isolation and laboratory cultivation.