JD3 Medium

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

JD3 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................4.0g
Papaic digest of soybean meal......................................................2.0g
Trisodium citrate...........................................................................2.0g
K 2 HPO 4 .........................................................................................1.5g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Disodium succinate.....................................................................0.01g
Bovine serum albumin solution.............................................100.0mL
Hemin chloride solution ..........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Bovine Serum Albumin Solution:
Composition per 100.0mL:
Bovine serum albumin fraction V.................................................2.0g
Preparation of Bovine Serum Albumin Solution: Add 2.0g of
bovine serum albumin fraction V to 100.0mL of distilled/deionized
water. Mix thoroughly. Filter sterilize.
Hemin Chloride Solution:
Composition per 10.0mL:
Hemin chloride ...........................................................................0.01g
Preparation of Hemin Chloride Solution: Add 0.01g of hemin
chloride to 10.0 mL of 0.5N NaOH. Mix thoroughly.
Preparation of Medium: Add components, except bovine serum
albumin solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Adjust to pH 7.0. Autoclave for 20 min at
15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL of ster-
ile bovine serum albumin solution. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks.
Use:  For the cultivation of ATCC strain 33107.