- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
培养基配方
您现在的位置： 网站首页 >> 培养基配方
ISP Medium 4 with Glucose (International Streptomyces Project Medium 4 with Glucose)
[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]
ISP Medium 4 with Glucose
(International Streptomyces Project Medium 4
with Glucose)
Glucose .......................................................................................20.0g
Soluble starch..............................................................................10.0g
CaCO 3 ...........................................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
NaCl..............................................................................................1.0g
FeSO 4 ·7H 2 O...............................................................................1.0mg
MnCl 2 ·7H 2 O...............................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling with frequent agitation. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
with swirling or leave in tubes.
Use: For the cultivation and maintenance of Streptomyces purpureus.
(International Streptomyces Project Medium 4
with Glucose)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................20.0gGlucose .......................................................................................20.0g
Soluble starch..............................................................................10.0g
CaCO 3 ...........................................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
NaCl..............................................................................................1.0g
FeSO 4 ·7H 2 O...............................................................................1.0mg
MnCl 2 ·7H 2 O...............................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling with frequent agitation. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
with swirling or leave in tubes.
Use: For the cultivation and maintenance of Streptomyces purpureus.
