ISP 5 Medium (DSMZ Medium 993)

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

ISP 5 Medium
(DSMZ Medium 993)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Glycerol ......................................................................................10.0g
L -asparagine, anhydrous ...............................................................1.0g
K 2 HPO 4 , anhydrous......................................................................1.0g
Trace elements solution .............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Distribute into tubes or flasks. Gently heat while stirring and bring to
boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into Petri dishes or leave in tubes.
Use: For the cultivation of Acrocarpospora macrocephala.