Isosphaera pallida Medium (DSMZ Medium 765)

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Isosphaera pallida Medium
(DSMZ Medium 765)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1005.0mL:

Solution A..............................................................................900.0mL
NaHCO 3  solution...................................................................100.0mL
Vitamin solution.........................................................................5.0mL
pH 7.9 ± 0.2 at 25°C
Solution A:
Composition per 900.0mL:
KCl............................................................................................... 4.0g
MgSO 4 ·7H 2 O................................................................................2.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
Glucose.......................................................................................0.25g
Casamino acids...........................................................................0.25g
CaCl 2 ·2H 2 O ..................................................................................0.2g
FeCl 3 ......................................................................................0.292mg
Trace elements solution SL-7a ..................................................1.0mL
Trace Elements Solution SL-7a:
CoCl 2 ·6H 2 O...........................................................................200.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................60.0mg
Na 2 MoO 4 ·4H 2 O.......................................................................40.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
CuCl 2 ·2H 2 O.............................................................................20.0mg

HCl (25%)..................................................................................1.0mL

Preparation of Trace Elements Solution SL-7a: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Sparge with 95%
air + 5% CO 2 .
Vitamin Solution:
Composition per 100.0mL:
Nicotinic acid...........................................................................20.0mg
Thiamine-HCl·2H 2 O................................................................10.0mg
p-Aminobenzoic acid.................................................................0.2mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................4.2g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge
with 80% N 2 + 20% CO 2 . Filter sterilize. Seal in serum bottle under
100% CO 2 .
Preparation of Medium: Dispense under 95% air + 5% CO 2 . Fill
serum bottles so that the gas to liquid ratio is about 5:1 (v/v). Sparge
with 95% air + 5% CO 2 . Seal bottles. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Using a syringe, inject
10.0mL sterile NaHCO 3 solution/90.0mL solution A. Then inject 0.5
mL sterile vitamin solution per 100.0mL of the resulting medium.
Use:  For the cultivation of Isosphaera pallida=Isocystis pallida.