Iso Sensitest Broth

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Iso Sensitest Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casein, hydrolyzed......................................................................11.0g
Peptones........................................................................................3.0g
NaCl..............................................................................................3.0g
Glucose .........................................................................................2.0g
Na 2 HPO 4 .......................................................................................2.0g
Sodium acetate..............................................................................1.0g
Soluble starch................................................................................1.0g
Magnesium glycerophosphate ......................................................0.2g
Calcium gluconate ........................................................................0.1g
L -cysteine·HCl ............................................................................0.02g
L- Tryptophan...............................................................................0.02g
Adenine.......................................................................................0.01g
Guanine.......................................................................................0.01g
Xanthine......................................................................................0.01g
Uracil ..........................................................................................0.01g
Nicotinamide..............................................................................3.0mg
Pantothenate...............................................................................3.0mg
Pyridoxine..................................................................................3.0mg
MnCl 2 ·4H 2 O...............................................................................2.0mg
CoSO 4 ........................................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
FeSO 4 ·7H 2 O...............................................................................1.0mg
Menadione .................................................................................1.0mg
Cyanocobalamin ........................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
Biotin .........................................................................................0.3mg
Thiamine..................................................................................0.04mg
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For antimicrobial susceptibility testing.