Iron-Oxidizing Medium

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Iron-Oxidizing Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 ....................................................................................3.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
KCl................................................................................................0.1g
Ca(NO 3 ) 2 .....................................................................................0.01g
FeSO 4 ·7H 2 O solution.............................................................300.0mL
H 2 SO 4 (10N)..............................................................................1.0mL
pH 3.0–3.6 at 25°C
FeSO 4 ·7H 2 O Solution:
Composition per 300.0mL:
FeSO 4 ·7H 2 O..............................................................................44.22g
Preparation of FeSO 4 ·7H 2 O Solution: Add FeSO 4 ·7H 2 O to dis-
tilled/deionized water and bring volume to 300.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except FeSO 4 ·7H 2 O so-
lution, to distilled/deionized water and bring volume to 700.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 25°C. Aseptically add 300.0mL of sterile
FeSO 4 ·7H 2 O solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.

Use: For the enumeration, isolation, and cultivation of iron and sulfur

bacteria, such as Thiobacillus ferrooxidans.